Displacement chromatography on synthetic ion-exchange resins. 4. The isolation of glucosamine and histidine from a protein hydrolysate
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منابع مشابه
Displacement Chromatography on Synthetic Ion - exchange Resins
In Part 1 of this series of papers (Partridge & Westall, 1949) it was shown that, whilst the baseexchange resin Zeo Karb 215 may be used with success for the separation of the neutral and acidic amino-acids, certain difficulties arise when the separation of basic substances is attempted. These difficulties were traced to the phenolic hydroxyl groups of the resin. Resins of the sulphonated pheno...
متن کاملDisplacement chromatography on synthetic ion-exchange resins. 1. Separation of organic bases and amino-acids using cation-exchange resins.
9. Accumulation of glutamate also occurred in isolated kidney cortex, spleen, lung and chorion. Six other tissues examined maintained a concentration gradient between tissue and medium, but did not increase the glutamate concentration above the original level. 10. It is pointed out that tissue slices afford a system in which problems of permeability and storage can be studied under controlled c...
متن کاملAmino acid sequence in lysozyme. I. Displacement chromatography of peptides from a partial hydrolysate on ion-exchange resins.
It has been shown (Fraenkel-Conrat, 1950) that the enzymic activity of lysozyme depends upon the presence of some of its amino, carboxyl and hydroxyl groups. A study of the structure of the lysozyme molecule as awhole has been undertaken in an approach to the problem of defining that portion of the molecule which is concerned in its activity. The lysozyme molecule probably consists of a single ...
متن کاملPreparative isolation of amino acids by carrier displacement chromatography on ion exchange resins.
Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
متن کاملPreparative Isolation of Amino Acids by Carrier Displacement Chromatography on Ion Exchange Resins* by Donald
Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1949
ISSN: 0306-3283
DOI: 10.1042/bj0450459